Narishige Pc10 Manual New File
A: The original PC-10 manual included a table correlating dial settings (e.g., 45–55 for 1mm borosilicate) to expected tip resistance (in MΩ). Without this table, you are guessing.
Narishige International (Narishige Group) still supports legacy products. While the PC-10 is discontinued, they often retain PDF archives.
| Feature | Narishige PC-10 | Sutter P-97 | HEKA PIP5 | |--------|----------------|-------------|------------| | Pulling cycle | Single gravity | Multi-cycle magnetic pull | Programmable solenoid | | Heating control | Analog dial | Digital PID | Digital | | Memory | None | 100 programs | 10 programs | | Pipette uniformity | Fair | Excellent | Very good | | Learning curve | Low (1 hour) | High (1 week) | Medium | | Price (new) | ~$2,500 | ~$8,500 | ~$6,000 | | Manual clarity | Good (with diagrams) | Dense | Excellent | narishige pc10 manual new
The PC-10 is not for complex patch-clamp configurations (e.g., steep taper for nucleated patches) but works fine for standard whole-cell and sharp electrodes.
The original PC-10 manual was written for 1980s lab conditions—stable mains voltage, mercury thermometers, and patience. The new community manuals assume you have a multimeter, a smartphone camera for macro shots, and maybe a 3D printer for replacement knobs. A: The original PC-10 manual included a table
In short: The "new" manual makes the old puller usable again.
Understanding how variables interact is key to mastering the PC-10. The original PC-10 manual was written for 1980s
| Symptom | Possible Cause | Solution | | :--- | :--- | :--- | | Glass does not separate | Temperature too low | Increase Heater Knob setting. | | | Heater coil broken | Inspect coil for continuity; replace if necessary. | | Tip is clogged / closed | Temperature too high / Pull too slow | Reduce heat to create a sharper break. | | | Dirty glass | Clean glass with alcohol or acetone before pulling. | | Tip is too wide/blunt | Pull was too fast (too hot) | Lower the heater temperature. | | | Weight too heavy | Use a lighter weight for a longer, finer taper. | | Tip is too long/whippy | Pull was too slow (too cool) | Increase heater temperature. | | | Weight too light | Use a heavier weight. | | Uneven tips | Glass not centered in coil | Adjust the glass holder to ensure the glass is perfectly vertical and centered. |
You have three legitimate options. Beware of scam sites offering "instant download" for a fee.
If you need a physical reprint or a professionally restored manual, try:
The most critical section of the PC-10 manual is the calibration protocol. This includes: