| Parameter | Expected Value | Improvement | | :--- | :--- | :--- | | Caffeine k' | 2.3 | 109% increase | | Aspirin Tailing | 1.3 | 46% reduction | | Aspirin Plates | 5,200 | 86% increase |

Instead of manual trial-and-error, you input compound properties (Log D, pKa), and the software predicts retention times. It then automatically writes the optimized HPLC program for your system.

  • Tip: Program a "pressure ramp" – gradual flow increase to prevent over-pressure alarms.

    • There is no single perfect HPLC program – only one that balances speed, resolution, and pressure. Here is how to tune your program for specific goals.

    | Vial Position | Sample ID | Injection Type | Run Time (min) | | :--- | :--- | :--- | :--- | | A1 | Blank (Mobile Phase) | Blank | 25 | | A2 | Standard – Level 1 | Calibration | 25 | | A3 | Standard – Level 2 | Calibration | 25 | | A4 | Standard – Level 3 | Calibration | 25 | | B1 | QC Sample | Quality Control | 25 | | B2 | Sample 001 | Unknown | 25 | | B3 | Sample 002 | Unknown | 25 | | B4 | Standard – Level 2 (Check) | Continuing Calibration | 25 |

    The current program utilizes a reversed-phase mechanism with the following parameters: